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KMID : 1144820220280040317
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대한의생명과학회지
2022 Volume.28 No. 4 p.317 ~ p.321
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Microbial Contamination according to the Numbers of Mask Worn in the Community
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Lee Eun-Ju
Park Hee-Chul
Je Min-A
Jung Song-hee
Myoung Ga-hee
Jo Su-Bin
Hwang Hyun-Min
Si Ryeong
Jin Hyun-Woo
Lee Kyung-Eun
Kim Jung-Ho
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Abstract
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Due to COVID-19 pandemic, wearing face masks is obligatory to prevent respiratory virus transmissions in the community. However, there are few studies of the desirable number of wearing a face mask, and how to store them for reuse. Therefore, in this study, a survey was conducted among 208 healthy adults, and 27 kf-94 masks worn for 1, 2, and 3 days were collected. To estimate the risk of bacterial contamination, we analyzed the extent of bacterial contamination of the BHI medium and 16S rRNA gene sequencing. With an increase in the number of days of using the mask, the degree of bacterial contamination of the used mask gradually increased. As a result of 16S rRNA PCR performed for strain identification, Staphylococcus, known as a pathogenic bacterium, was identified the most. In conclusion, we found that wearing a cotton KF mask provides an optimal environment for microbes, which are related to the skin and respiratory system, to thrive. Therefore, it is also important to reduce the risk of bacterial infection of the face mask with appropriate sterilization methods.
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KEYWORD
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Face masks, COVID-19, Microbiome contamination, Bacterial 16S rRNA sequencing
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